The present invention is related to the design of haptens, structurally related to pyocyanin, a toxin secreted by the Gram negative bacterium Pseudomonas aeruginosa, and its derivatives. It is also related to the hapten conjugates used for the production of specific antibodies against such substances. Furthermore, the invention refers to a method and a kit for the detection and quantification of pyocyanin and its derivatives, using the mentioned antibodies, to detect infections due to Pseudomonas aeruginosa.
- Design of immunizing haptens, capable of generating specific antibodies
- Hihgly specific and sensitive tests, decreasing detection threshold
- Quick diagnostic tests, shortening time for pathogen identification
- Automated, miniaturized, and non-invasive tests
- Tests require a small volume of samples, easily obtainable.
- Neither specialized personnel nor clinical laboratory needed
To be determined during further development.
- Detection of Pseudomonas aeruginosa infections
- Quantification of 1-hydroxyphenazin and pyocyanin in subject samples
- Immunoassays (ELISA, lateral-flow immunoassay, Western or dot blotting, immunoturbidimetry, immunosensor, immunosorbents, immunoprecipitation, radioimmunoassay, immunofluorescence, immunohistochemistry, flow cytometry, etc.)
- Immunoaffinity extraction systems (chromatography, biofunctionalized particles, etc.)
Aida Castellanos
